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mouse anti human osteocalcin monoclonal antibody  (R&D Systems)


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    R&D Systems mouse anti human osteocalcin monoclonal antibody
    FD osteoblasts exhibit defective phenotype. ALP quantification ( A ) and staining ( B ). Calcium concentration ( C ) and Alizarin Red staining ( D ). Scale bar: 75 μm ( B , D ); COL1A1 ( E ) and BGLAP ( F ) gene expression measurement by RT-qPCR. ( G ) Representative photographs of FD and non-FD cell cultures exhibit different type-I collagen and <t>osteocalcin</t> expression at D21 of culture. Type-I collagen (a, b) and Osteocalcin (c, d) immunostainings. Colorized overlayed images (e, f): ColIa1 (AlexaFluor488)/OC (AlexaFluor568)/nucleus (DAPI). Grey values represent non-FD osteoblasts and black values represent FD osteoblasts. Images were acquired with a Zeiss Axiovert 200 M inverted microscope. Scale bar: 20 μm (G: a,b, c,d, e,f). FD, n = 7; non-FD, n = 8. Δ p < 0.05 FD versus non-FD; # p < 0.05 versus D7; £ p < 0.05 versus D14
    Mouse Anti Human Osteocalcin Monoclonal Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 69 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse anti human osteocalcin monoclonal antibody/product/R&D Systems
    Average 95 stars, based on 69 article reviews
    mouse anti human osteocalcin monoclonal antibody - by Bioz Stars, 2026-04
    95/100 stars

    Images

    1) Product Images from "In vitro characterization of osteoblasts from craniofacial fibrous dysplasia of bone and their impact on bone homeostasis"

    Article Title: In vitro characterization of osteoblasts from craniofacial fibrous dysplasia of bone and their impact on bone homeostasis

    Journal: Orphanet Journal of Rare Diseases

    doi: 10.1186/s13023-026-04262-0

    FD osteoblasts exhibit defective phenotype. ALP quantification ( A ) and staining ( B ). Calcium concentration ( C ) and Alizarin Red staining ( D ). Scale bar: 75 μm ( B , D ); COL1A1 ( E ) and BGLAP ( F ) gene expression measurement by RT-qPCR. ( G ) Representative photographs of FD and non-FD cell cultures exhibit different type-I collagen and osteocalcin expression at D21 of culture. Type-I collagen (a, b) and Osteocalcin (c, d) immunostainings. Colorized overlayed images (e, f): ColIa1 (AlexaFluor488)/OC (AlexaFluor568)/nucleus (DAPI). Grey values represent non-FD osteoblasts and black values represent FD osteoblasts. Images were acquired with a Zeiss Axiovert 200 M inverted microscope. Scale bar: 20 μm (G: a,b, c,d, e,f). FD, n = 7; non-FD, n = 8. Δ p < 0.05 FD versus non-FD; # p < 0.05 versus D7; £ p < 0.05 versus D14
    Figure Legend Snippet: FD osteoblasts exhibit defective phenotype. ALP quantification ( A ) and staining ( B ). Calcium concentration ( C ) and Alizarin Red staining ( D ). Scale bar: 75 μm ( B , D ); COL1A1 ( E ) and BGLAP ( F ) gene expression measurement by RT-qPCR. ( G ) Representative photographs of FD and non-FD cell cultures exhibit different type-I collagen and osteocalcin expression at D21 of culture. Type-I collagen (a, b) and Osteocalcin (c, d) immunostainings. Colorized overlayed images (e, f): ColIa1 (AlexaFluor488)/OC (AlexaFluor568)/nucleus (DAPI). Grey values represent non-FD osteoblasts and black values represent FD osteoblasts. Images were acquired with a Zeiss Axiovert 200 M inverted microscope. Scale bar: 20 μm (G: a,b, c,d, e,f). FD, n = 7; non-FD, n = 8. Δ p < 0.05 FD versus non-FD; # p < 0.05 versus D7; £ p < 0.05 versus D14

    Techniques Used: Staining, Concentration Assay, Gene Expression, Quantitative RT-PCR, Expressing, Inverted Microscopy



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    Image Search Results


    FD osteoblasts exhibit defective phenotype. ALP quantification ( A ) and staining ( B ). Calcium concentration ( C ) and Alizarin Red staining ( D ). Scale bar: 75 μm ( B , D ); COL1A1 ( E ) and BGLAP ( F ) gene expression measurement by RT-qPCR. ( G ) Representative photographs of FD and non-FD cell cultures exhibit different type-I collagen and osteocalcin expression at D21 of culture. Type-I collagen (a, b) and Osteocalcin (c, d) immunostainings. Colorized overlayed images (e, f): ColIa1 (AlexaFluor488)/OC (AlexaFluor568)/nucleus (DAPI). Grey values represent non-FD osteoblasts and black values represent FD osteoblasts. Images were acquired with a Zeiss Axiovert 200 M inverted microscope. Scale bar: 20 μm (G: a,b, c,d, e,f). FD, n = 7; non-FD, n = 8. Δ p < 0.05 FD versus non-FD; # p < 0.05 versus D7; £ p < 0.05 versus D14

    Journal: Orphanet Journal of Rare Diseases

    Article Title: In vitro characterization of osteoblasts from craniofacial fibrous dysplasia of bone and their impact on bone homeostasis

    doi: 10.1186/s13023-026-04262-0

    Figure Lengend Snippet: FD osteoblasts exhibit defective phenotype. ALP quantification ( A ) and staining ( B ). Calcium concentration ( C ) and Alizarin Red staining ( D ). Scale bar: 75 μm ( B , D ); COL1A1 ( E ) and BGLAP ( F ) gene expression measurement by RT-qPCR. ( G ) Representative photographs of FD and non-FD cell cultures exhibit different type-I collagen and osteocalcin expression at D21 of culture. Type-I collagen (a, b) and Osteocalcin (c, d) immunostainings. Colorized overlayed images (e, f): ColIa1 (AlexaFluor488)/OC (AlexaFluor568)/nucleus (DAPI). Grey values represent non-FD osteoblasts and black values represent FD osteoblasts. Images were acquired with a Zeiss Axiovert 200 M inverted microscope. Scale bar: 20 μm (G: a,b, c,d, e,f). FD, n = 7; non-FD, n = 8. Δ p < 0.05 FD versus non-FD; # p < 0.05 versus D7; £ p < 0.05 versus D14

    Article Snippet: Cells were permeabilized with 3% BSA (Sigma Aldrich, Saint-Quentin Fallavier, France) plus 0.1% Triton X100 (Acros Organics, Thermo Fisher Scientific, Illkirch, France) for 45 min then incubated overnight at 4 °C with rabbit anti-human type-I collagen monoclonal antibody (clone H-197, Santa Cruz Biotechnology, Heidelberg, Germany) and mouse anti-human osteocalcin monoclonal antibody (clone 190125, R&D systems, Lille, France).

    Techniques: Staining, Concentration Assay, Gene Expression, Quantitative RT-PCR, Expressing, Inverted Microscopy

    Biological testing results with KUSA-A1 and E. coli : ( a ) KUSA-A1 cell optical density, ( b ) area coverage of the osteocalcin staining with respect to the investigated area of the sample, ( c ) E. coli optical density. Results marked with “*” have a p -value < 0.05 when compared with all others.

    Journal: Polymers

    Article Title: Simple Electrospinning Method for Biocompatible Polycaprolactone β-Carotene Scaffolds: Advantages and Limitations

    doi: 10.3390/polym16101371

    Figure Lengend Snippet: Biological testing results with KUSA-A1 and E. coli : ( a ) KUSA-A1 cell optical density, ( b ) area coverage of the osteocalcin staining with respect to the investigated area of the sample, ( c ) E. coli optical density. Results marked with “*” have a p -value < 0.05 when compared with all others.

    Article Snippet: Osteogenic differentiation was assessed after 10 days in the osteogenic medium by staining cultured KUSA-A1 cells with the green osteocalcin stain (Osteocalcin Mouse anti-Human, PE, Clone, Fisher Scientific, Hampton, NH, USA) following the manufacturer’s instructions.

    Techniques: Staining